Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli.
Recombinant DNA plasmids containing the gene for the membrane-bound D-lactate dehydrogenase (D-LDH) of Escherichia coli linked to the promoter PL from lambda were constructed. After induction, the levels of D-LDH were elevated 300-fold over that of the wild type and amounted to 35% of the total cell...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1985
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215007/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3882663/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.3.1059-1068.1985 |
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