Overproduction and nucleotide sequence of the respiratory D-lactate dehydrogenase of Escherichia coli.

Recombinant DNA plasmids containing the gene for the membrane-bound D-lactate dehydrogenase (D-LDH) of Escherichia coli linked to the promoter PL from lambda were constructed. After induction, the levels of D-LDH were elevated 300-fold over that of the wild type and amounted to 35% of the total cell...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Rule, G S, Pratt, E A, Chin, C C, Wold, F, Ho, C
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1985
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC215007/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3882663/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.3.1059-1068.1985
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