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Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains.

Lipopolysaccharide-defective mutants of Salmonella typhimurium were transformed by plasmid DNA with a Ca2+ treatment method. Only those mutants with an Rc or Rd2 chemotype, due to galE or rfaF mutations, respectively, gave efficiencies greater than 10(5) transformants per microgram of DNA, frequenci...

Ausführliche Beschreibung

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Bibliografische Detailangaben
Veröffentlicht in:J Bacteriol
Hauptverfasser: MacLachlan, P R, Sanderson, K E
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1985
Schlagworte:
Online-Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214893/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3881397/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.1.442-445.1985
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