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Transformation of Salmonella typhimurium with plasmid DNA: differences between rough and smooth strains.

Lipopolysaccharide-defective mutants of Salmonella typhimurium were transformed by plasmid DNA with a Ca2+ treatment method. Only those mutants with an Rc or Rd2 chemotype, due to galE or rfaF mutations, respectively, gave efficiencies greater than 10(5) transformants per microgram of DNA, frequenci...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: MacLachlan, P R, Sanderson, K E
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1985
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214893/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3881397/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.1.442-445.1985
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