Isolation of a versatile Serratia marcescens mutant as a host and molecular cloning of the aspartase gene.
An extracellular nuclease-deficient, antibiotic-sensitive, and restrictionless mutant was isolated from the wild-type strain of Serratia marcescens Sr41 by four rounds of mutagenesis. The mutant was transformed efficiently with plasmid DNAs prepared from Escherichia coli and S. marcescens, and was u...
Guardado en:
| Publicado en: | J Bacteriol |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1985
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214826/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2981795/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.1.1-6.1985 |
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