Isolation of a versatile Serratia marcescens mutant as a host and molecular cloning of the aspartase gene.
An extracellular nuclease-deficient, antibiotic-sensitive, and restrictionless mutant was isolated from the wild-type strain of Serratia marcescens Sr41 by four rounds of mutagenesis. The mutant was transformed efficiently with plasmid DNAs prepared from Escherichia coli and S. marcescens, and was u...
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| 發表在: | J Bacteriol |
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| Principais autores: | , |
| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
American Society for Microbiology (ASM)
1985
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214826/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2981795/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.161.1.1-6.1985 |
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