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Vegetative expression of the delta-endotoxin genes of Bacillus thuringiensis subsp. kurstaki in Bacillus subtilis.

Bacillus thuringiensis subsp. kurstaki total DNA was digested with BglII and cloned into the BamHI site of plasmid pUC9 in Escherichia coli. A recombinant plasmid, pHBHE, expressed a protein of 135,000 daltons that was toxic to caterpillars. A HincII-SmaI double digest of pHBHE was then ligated to B...

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Published in:J Bacteriol
Main Authors: Shivakumar, A G, Gundling, G J, Benson, T A, Casuto, D, Miller, M F, Spear, B B
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1986
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214576/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3007434/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.166.1.194-204.1986
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