Vegetative expression of the delta-endotoxin genes of Bacillus thuringiensis subsp. kurstaki in Bacillus subtilis.

Bacillus thuringiensis subsp. kurstaki total DNA was digested with BglII and cloned into the BamHI site of plasmid pUC9 in Escherichia coli. A recombinant plasmid, pHBHE, expressed a protein of 135,000 daltons that was toxic to caterpillars. A HincII-SmaI double digest of pHBHE was then ligated to B...

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Main Authors: Shivakumar, A G, Gundling, G J, Benson, T A, Casuto, D, Miller, M F, Spear, B B
Formato: Artigo
Idioma:Inglês
Publicado: 1986
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC214576/
https://ncbi.nlm.nih.gov/pubmed/3007434
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