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Genetic analysis of Bacillus stearothermophilus by protoplast fusion.

Efficient and reliable protoplasting, regeneration, and fusion techniques were established for the prototrophic strain Bacillus stearothermophilus NUB36. Auxotrophic mutants were isolated, and protoplast fusion was used to construct isogenic mutant strains and for chromosomal mapping. Markers were m...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Chen, Z F, Wojcik, S F, Welker, N E
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214527/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3512533/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.165.3.994-1001.1986
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