Genetic analysis of Bacillus stearothermophilus by protoplast fusion.

Efficient and reliable protoplasting, regeneration, and fusion techniques were established for the prototrophic strain Bacillus stearothermophilus NUB36. Auxotrophic mutants were isolated, and protoplast fusion was used to construct isogenic mutant strains and for chromosomal mapping. Markers were m...

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Pubblicato in:	J Bacteriol
Autori principali:	Chen, Z F, Wojcik, S F, Welker, N E
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1986
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214527/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3512533/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.165.3.994-1001.1986
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