NMR study of Ni2+ binding to the H-N-H endonuclease domain of colicin E9.

Ni2+ affinity columns are widely used for protein purification, but they carry the risk that Ni2+ ions may bind to the protein, either adventitiously or at a physiologically important site. Dialysis against ethylenediaminetetraacetic acid (EDTA) is normally used to remove metal ions bound adventitio...

Cijeli opis

Spremljeno u:
Bibliografski detalji
Glavni autori: Hannan, J. P., Whittaker, S. B., Davy, S. L., Kühlmann, U. C., Pommer, A. J., Hemmings, A. M., James, R., Kleanthous, C., Moore, G. R.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 1999
Teme:
Research Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144424/
https://ncbi.nlm.nih.gov/pubmed/10452617
Oznake: Dodaj oznaku
Bez oznaka, Budi prvi tko označuje ovaj zapis!

Similar Items