NMR study of Ni2+ binding to the H-N-H endonuclease domain of colicin E9.

Ni2+ affinity columns are widely used for protein purification, but they carry the risk that Ni2+ ions may bind to the protein, either adventitiously or at a physiologically important site. Dialysis against ethylenediaminetetraacetic acid (EDTA) is normally used to remove metal ions bound adventitio...

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Autores principales: Hannan, J. P., Whittaker, S. B., Davy, S. L., Kühlmann, U. C., Pommer, A. J., Hemmings, A. M., James, R., Kleanthous, C., Moore, G. R.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 1999
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144424/
https://ncbi.nlm.nih.gov/pubmed/10452617
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