Cloning, mutagenesis, and structural analysis of human pancreatic alpha-amylase expressed in Pichia pastoris.

Human pancreatic alpha-amylase (HPA) was expressed in the methylotrophic yeast Pichia pastoris and two mutants (D197A and D197N) of a completely conserved active site carboxylic acid were generated. All recombinant proteins were shown by electrospray ionization mass spectrometry (ESI-MS) to be glyco...

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Main Authors: Rydberg, E. H., Sidhu, G., Vo, H. C., Hewitt, J., Côte, H. C., Wang, Y., Numao, S., MacGillivray, R. T., Overall, C. M., Brayer, G. D., Withers, S. G.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 1999
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144294/
https://ncbi.nlm.nih.gov/pubmed/10091666
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