Gateway cloning is compatible with protein secretion from Pichia pastoris

Secretion of a recombinant protein from the yeast Pichia pastoris requires the presence of a signal peptide at the amino terminus. Maintaining the full amino acid sequence of the signal peptide is thought to be important for proper signal processing and protein secretion. We show that at least for o...

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Bibliographic Details
Published in:Protein Expr Purif
Main Authors: Esposito, Dominic, Gillette, William K., Miller, David A., Taylor, Troy E., Frank, Peter H., Hu, Renqui, Bekisz, Joseph, Hernandez, Jessica, Cregg, James M., Zoon, Kathryn C., Hartley, James L.
Format: Artigo
Language:Inglês
Published: Elsevier Inc. 2005
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7130006/
https://ncbi.nlm.nih.gov/pubmed/15766886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2004.12.006
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