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Gateway cloning is compatible with protein secretion from Pichia pastoris

Secretion of a recombinant protein from the yeast Pichia pastoris requires the presence of a signal peptide at the amino terminus. Maintaining the full amino acid sequence of the signal peptide is thought to be important for proper signal processing and protein secretion. We show that at least for o...

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書目詳細資料
發表在:Protein Expr Purif
Main Authors: Esposito, Dominic, Gillette, William K., Miller, David A., Taylor, Troy E., Frank, Peter H., Hu, Renqui, Bekisz, Joseph, Hernandez, Jessica, Cregg, James M., Zoon, Kathryn C., Hartley, James L.
格式: Artigo
語言:Inglês
出版: Elsevier Inc. 2005
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC7130006/
https://ncbi.nlm.nih.gov/pubmed/15766886
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2004.12.006
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