Limited proteolysis of bovine alpha-lactalbumin: isolation and characterization of protein domains.

The partly folded states of alpha-lactalbumin (alpha-LA) exposed to acid solution at pH 2.0 (A-state) or at neutral pH upon EDTA-mediated removal of the single protein-bound calcium ion (apo form) have been probed by limited proteolysis experiments. These states are nowadays commonly considered to b...

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Autors principals: Polverino de Laureto, P., Scaramella, E., Frigo, M., Wondrich, F. G., De Filippis, V., Zambonin, M., Fontana, A.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 1999
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144187/
https://ncbi.nlm.nih.gov/pubmed/10595532
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