Limited proteolysis of ribonuclease A with thermolysin in trifluoroethanol.

We have examined the proteolysis of bovine pancreatic ribonuclease A (RNase) by thermolysin when dissolved in aqueous buffer, pH 7.0, in the presence of 50% (v/v) trifluoroethanol (TFE). Under these solvent conditions, RNase acquires a conformational state characterized by an enhanced content of sec...

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Detaylı Bibliyografya
Asıl Yazarlar: Polverino de Laureto, P., Scaramella, E., De Filippis, V., Bruix, M., Rico, M., Fontana, A.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Cold Spring Harbor Laboratory Press 1997
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144764/
https://ncbi.nlm.nih.gov/pubmed/9098896
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