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Purification and characterization of a cobalt-activated carboxypeptidase from the hyperthermophilic archaeon Pyrococcus furiosus.

A novel metallocarboxypeptidase (PfuCP) has been purified to homogeneity from the hyperthermophilic archaeon, Pyrococcus furiosus, with its intended use in C-terminal ladder sequencing of proteins and peptides at elevated temperatures. PfuCP was purified in its inactive state by the addition of ethy...

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Bibliografske podrobnosti
Main Authors: Cheng, T. C., Ramakrishnan, V., Chan, S. I.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 1999
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144183/
https://ncbi.nlm.nih.gov/pubmed/10595552
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