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Reactivation of thermally inactivated pre-beta-lactamase by DnaK, DnaJ, and GrpE.

To understand the role of the 23-amino acid signal sequence in the folding and stability of beta-lactamase, the precursor and a mutant beta-lactamase with a 19-amino acid signal sequence deletion were synthesized in vitro using an Escherichia coli cell-free coupled transcription/translation system....

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Autors principals: McCarthy, D., Kramer, G., Hardesty, B.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 1998
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144002/
https://ncbi.nlm.nih.gov/pubmed/9605320
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