Reactivation of thermally inactivated pre-beta-lactamase by DnaK, DnaJ, and GrpE.

To understand the role of the 23-amino acid signal sequence in the folding and stability of beta-lactamase, the precursor and a mutant beta-lactamase with a 19-amino acid signal sequence deletion were synthesized in vitro using an Escherichia coli cell-free coupled transcription/translation system....

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Bibliographic Details
Main Authors: McCarthy, D., Kramer, G., Hardesty, B.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 1998
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2144002/
https://ncbi.nlm.nih.gov/pubmed/9605320
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