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A monomeric mutant of Clostridium symbiosum glutamate dehydrogenase: comparison with a structured monomeric intermediate obtained during refolding.

The refolding of Clostridium symbiosum glutamate dehydrogenase (GDH) involves the formation of an inactive structured monomeric intermediate prior to its concentration-dependent association. The structured monomer obtained after removal of guanidinium chloride was stable and competent for reconstitu...

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Autori principali: Millevoi, S., Pasquo, A., Chiaraluce, R., Consalvi, V., Giangiacomo, L., Britton, K. L., Stillman, T. J., Rice, D. W., Engel, P. C.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Cold Spring Harbor Laboratory Press 1998
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143975/
https://ncbi.nlm.nih.gov/pubmed/9568903
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