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Semisynthesis of cytotoxic proteins using a modified protein splicing element.

Two cytotoxic proteins, bovine pancreatic ribonuclease A (RNase A), and a restriction endonuclease from Haemophilus parainfluenzae (HpaI), were produced using a novel semisynthetic approach that utilizes a protein splicing element, an intein, to generate a reactive thioester at the C-terminus of a r...

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Main Authors: Evans, T. C., Benner, J., Xu, M. Q.
格式: Artigo
語言:Inglês
出版: Cold Spring Harbor Laboratory Press 1998
主題:
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143866/
https://ncbi.nlm.nih.gov/pubmed/9827992
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