Preparation and properties of pure, full-length IclR protein of Escherichia coli. Use of time-of-flight mass spectrometry to investigate the problems encountered.

IclR protein, the repressor of the aceBAK operon of Escherichia coli, has been examined by time-of-flight mass spectrometry, with ionization by matrix assisted laser desorption or by electrospray. The purified protein was found to have a smaller mass than that predicted from the base sequence of the...

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Autores principales: Donald, L. J., Chernushevich, I. V., Zhou, J., Verentchikov, A., Poppe-Schriemer, N., Hosfield, D. J., Westmore, J. B., Ens, W., Duckworth, H. W., Standing, K. G.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 1996
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143496/
https://ncbi.nlm.nih.gov/pubmed/8844850
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