Probing the oligomeric structure of an enzyme by electrospray ionization time-of-flight mass spectrometry.

Electrospray ionization time-of-flight (ESI-TOF) mass spectrometry was used to study the quaternary structure of 4-oxalocrotonate tautomerase (EC 5.3.2; 4OT), and four analogues prepared by total chemical synthesis. Wild-type 4OT is a hexamer of 62 amino acid subunits and contains no cysteine residu...

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发表在:	Proc Natl Acad Sci U S A
Principais autores:	Fitzgerald, M C, Chernushevich, I, Standing, K G, Whitman, C P, Kent, S B
格式:	Artigo
语言:	Inglês
出版:	National Academy of Sciences 1996
主题:	Research Article
在线阅读:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC38896/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8692908/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.14.6851
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