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Ala-insertion scanning mutagenesis of the glycophorin A transmembrane helix: a rapid way to map helix-helix interactions in integral membrane proteins.

Alanine insertions into the glycophorin A transmembrane helix are found to disrupt helix-helix dimerization in a way that is fully consistent with earlier saturation mutagenesis data, suggesting that Ala-insertion scanning can be used to rapidly map the approximate location of structurally and/or fu...

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Autors principals: Mingarro, I., Whitley, P., Lemmon, M. A., von Heijne, G.
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 1996
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2143459/
https://ncbi.nlm.nih.gov/pubmed/8819166
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