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Export defect adjacent to the processing site of staphylococcal nuclease is suppressed by a prlA mutation.

Plasmids have been constructed in which the Escherichia coli alkaline phosphatase promoter and signal sequence have been fused to the staphylococcal nuclease gene to promote the high-level expression and secretion of this gene product in E. coli. We determined that the first amino acid residue after...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Liss, L R, Johnson, B L, Oliver, D B
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1985
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214342/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3902802/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.164.2.925-928.1985
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