Export defect adjacent to the processing site of staphylococcal nuclease is suppressed by a prlA mutation.
Plasmids have been constructed in which the Escherichia coli alkaline phosphatase promoter and signal sequence have been fused to the staphylococcal nuclease gene to promote the high-level expression and secretion of this gene product in E. coli. We determined that the first amino acid residue after...
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| Publicado no: | J Bacteriol |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1985
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214342/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3902802/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.164.2.925-928.1985 |
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