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Characterization of the stable, acid-induced, molten globule-like state of staphylococcal nuclease.

Titration of a salt-free solution of native staphylococcal nuclease by HCl leads to an unfolding transition in the vicinity of pH 4, as determined by near- and far-UV circular dichroism. At pH 2-3, the protein is substantially unfolded. The addition of further HCl results in a second transition, thi...

詳細記述

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書誌詳細
主要な著者: Fink, A. L., Calciano, L. J., Goto, Y., Nishimura, M., Swedberg, S. A.
フォーマット: Artigo
言語:Inglês
出版事項: Cold Spring Harbor Laboratory Press 1993
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142415/
https://ncbi.nlm.nih.gov/pubmed/8358298
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