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Controlling the regiospecificity and coupling of cytochrome P450cam: T185F mutant increases coupling and abolishes 3-hydroxynorcamphor product.

Cytochrome P450cam (P450CIA1) catalyzes the hydroxylation of camphor and several substrate analogues such as norcamphor and 1-methyl-norcamphor. Hydroxylation was found experimentally at the 3, 5, and 6 positions of norcamphor, but only at the 5 and 6 positions of 1-methyl-norcamphor. In the catalyt...

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Bibliografske podrobnosti
Main Authors: Paulsen, M. D., Filipovic, D., Sligar, S. G., Ornstein, R. L.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 1993
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142384/
https://ncbi.nlm.nih.gov/pubmed/8453374
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