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Resolution of the fluorescence equilibrium unfolding profile of trp aporepressor using single tryptophan mutants.

Single tryptophan mutants of the trp aporepressor, tryptophan 19-->phenylalanine (W19F) and tryptophan 99-->phenylalanine (W99F), were used in this study to resolve the individual steady-state and time-resolved fluorescence urea unfolding profiles of the two tryptophan residues in this highly...

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Bibliografiske detaljer
Main Authors: Royer, C. A., Mann, C. J., Matthews, C. R.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 1993
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142281/
https://ncbi.nlm.nih.gov/pubmed/8268795
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