Purification and characterization of trp aporepressor.

We have isolated homogeneous trp aporepressor from an overproducing strain of Escherichia coli carrying a plasmid containing trpR preceded by tandem trp operon promoters. Dye-affinity and ion-exchange chromatography were used in conjunction with a gel electrophoresis assay in which the repressor, wh...

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Publié dans:	Proc Natl Acad Sci U S A
Auteurs principaux:	Joachimiak, A, Kelley, R L, Gunsalus, R P, Yanofsky, C, Sigler, P B
Format:	Artigo
Langue:	Inglês
Publié:	National Academy of Sciences 1983
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC393440/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6338493/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.3.668
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