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Purification and characterization of trp aporepressor.

We have isolated homogeneous trp aporepressor from an overproducing strain of Escherichia coli carrying a plasmid containing trpR preceded by tandem trp operon promoters. Dye-affinity and ion-exchange chromatography were used in conjunction with a gel electrophoresis assay in which the repressor, wh...

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Detalhes bibliográficos
Main Authors: Joachimiak, A, Kelley, R L, Gunsalus, R P, Yanofsky, C, Sigler, P B
Formato: Artigo
Idioma:Inglês
Publicado em: 1983
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC393440/
https://ncbi.nlm.nih.gov/pubmed/6338493
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