Substrate polarization by residues in delta 5-3-ketosteroid isomerase probed by site-directed mutagenesis and UV resonance Raman spectroscopy.

delta 5-3-Ketosteroid isomerase (KSI: EC 5.3.3.1) of Pseudomonas testosteroni catalyzes the isomerization of delta 5-3-ketosteroids to delta 4-3-ketosteroids by the stereospecific transfer of the steroid 4 beta-proton to the 6 beta-position, using Tyr-14 as a general acid and Asp-38 as a base. Ultra...

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Bibliographic Details
Main Authors: Austin, J. C., Kuliopulos, A., Mildvan, A. S., Spiro, T. G.
Format: Artigo
Language:Inglês
Published: Cold Spring Harbor Laboratory Press 1992
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2142197/
https://ncbi.nlm.nih.gov/pubmed/1339027
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