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Cis proline mutants of ribonuclease A. I. Thermal stability.
A chemically synthesized gene for ribonuclease A has been expressed in Escherichia coli using a T7 expression system (Studier, F.W., Rosenberg, A.H., Dunn, J.J., & Dubendorff, J.W., 1990, Methods Enzymol. 185, 60-89). The expressed protein, which contains an additional N-terminal methionine resi...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Cold Spring Harbor Laboratory Press
1992
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2142151/ https://ncbi.nlm.nih.gov/pubmed/1338975 |
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