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Determination of transmembrane topology of an inward-rectifying potassium channel from Arabidopsis thaliana based on functional expression in Escherichia coli

We report here that the inward-rectifying potassium channels KAT1 and AKT2 were functionally expressed in K(+) uptake-deficient Escherichia coli. Immunological assays showed that KAT1 was translocated into the cell membrane of E. coli. Functional assays suggested that KAT1 was inserted topologically...

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Main Authors: Uozumi, Nobuyuki, Nakamura, Tatsunosuke, Schroeder, Julian I., Muto, Shoshi
Format: Artigo
Jezik:Inglês
Izdano: The National Academy of Sciences 1998
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC21412/
https://ncbi.nlm.nih.gov/pubmed/9707551
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