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Determination of transmembrane topology of an inward-rectifying potassium channel from Arabidopsis thaliana based on functional expression in Escherichia coli

We report here that the inward-rectifying potassium channels KAT1 and AKT2 were functionally expressed in K(+) uptake-deficient Escherichia coli. Immunological assays showed that KAT1 was translocated into the cell membrane of E. coli. Functional assays suggested that KAT1 was inserted topologically...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Uozumi, Nobuyuki, Nakamura, Tatsunosuke, Schroeder, Julian I., Muto, Shoshi
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC21412/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9707551/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.17.9773
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