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Method for localization of cloned DNA fragments on the Escherichia coli chromosome.

In exponentially growing cultures of Escherichia coli strains carrying the dnaC28 mutation, DNA replication can be synchronized by temperature changes (R. L. Rodriguez, M. S. Dalbey, and C. I. Davern, J. Mol. Biol. 74:599-604, 1973). We used this synchronization procedure and DNA-DNA hybridization t...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Fayet, O, Prere, M F
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1987
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC214021/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3316190/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5641-5647.1987
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