Increased expression in Escherichia coli of a synthetic gene encoding human somatomedin C after gene duplication and fusion.

A synthetic gene coding for human somatomedin C (SMC) was inserted into an Escherichia coli plasmid vector that contains the bacteriophage lambda pL promoter. Intracellular accumulation of the gene product after induction of the promoter was found to be low. A 200-fold greater yield was obtained wit...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Schulz, M F, Buell, G, Schmid, E, Movva, R, Selzer, G
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1987
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213962/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3316181/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5385-5392.1987
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