Increased expression in Escherichia coli of a synthetic gene encoding human somatomedin C after gene duplication and fusion.

A synthetic gene coding for human somatomedin C (SMC) was inserted into an Escherichia coli plasmid vector that contains the bacteriophage lambda pL promoter. Intracellular accumulation of the gene product after induction of the promoter was found to be low. A 200-fold greater yield was obtained wit...

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-д хэвлэсэн:	J Bacteriol
Үндсэн зохиолчид:	Schulz, M F, Buell, G, Schmid, E, Movva, R, Selzer, G
Формат:	Artigo
Хэл сонгох:	Inglês
Хэвлэсэн:	American Society for Microbiology (ASM) 1987
Нөхцлүүд:	Research Article
Онлайн хандалт:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC213962/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3316181/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.12.5385-5392.1987
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