P1 plasmid replication: measurement of initiator protein concentration in vivo.
To study the functions of the mini-P1 replication initiation protein RepA quantitatively, we have developed a method to measure RepA concentration by using immunoblotting. In vivo, there are about 20 RepA dimers per unit-copy plasmid DNA. RepA was deduced to be a dimer from gel filtration of the pur...
সংরক্ষণ করুন:
| প্রকাশিত: | J Bacteriol |
|---|---|
| প্রধান লেখক: | , , , , |
| বিন্যাস: | Artigo |
| ভাষা: | Inglês |
| প্রকাশিত: |
American Society for Microbiology (ASM)
1987
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| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212459/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3611028/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.8.3737-3742.1987 |
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