P1 plasmid replication: measurement of initiator protein concentration in vivo.

To study the functions of the mini-P1 replication initiation protein RepA quantitatively, we have developed a method to measure RepA concentration by using immunoblotting. In vivo, there are about 20 RepA dimers per unit-copy plasmid DNA. RepA was deduced to be a dimer from gel filtration of the pur...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Swack, J A, Pal, S K, Mason, R J, Abeles, A L, Chattoraj, D K
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1987
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212459/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3611028/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.8.3737-3742.1987
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