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Initiation of DNA replication in nuclei from quiescent cells requires permeabilization of the nuclear membrane

We have investigated the replication capacity of intact nuclei from quiescent cells using Xenopus egg extract. Nuclei, with intact nuclear membranes, were isolated from both exponentially growing and contact- inhibited BALB/c 3T3 fibroblasts by treatment of the cells with streptolysin-O. Flow cytome...

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Natura: Artigo
Lingua:Inglês
Pubblicazione: The Rockefeller University Press 1994
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2120183/
https://ncbi.nlm.nih.gov/pubmed/7929570
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