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Initiation of DNA replication in nuclei from quiescent cells requires permeabilization of the nuclear membrane

We have investigated the replication capacity of intact nuclei from quiescent cells using Xenopus egg extract. Nuclei, with intact nuclear membranes, were isolated from both exponentially growing and contact- inhibited BALB/c 3T3 fibroblasts by treatment of the cells with streptolysin-O. Flow cytome...

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Detalhes bibliográficos
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 1994
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2120183/
https://ncbi.nlm.nih.gov/pubmed/7929570
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