Cloning and expression in Escherichia coli of a cellulase gene from Ruminococcus flavefaciens.

An endoglucanase gene of Ruminococcus flavefaciens FD1 was cloned on the vector pEcoR251 to form the recombinant plasmid pMEB200. The cloned endoglucanase gene showed carboxymethylcellulase enzyme activity but no degradation of Avicel (FMC Corp., Philadelphia, Pa.) or filter paper. Carboxymethylcell...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Barros, M E, Thomson, J A
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1987
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212014/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3549703/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.4.1760-1762.1987
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