Cloning and expression in Escherichia coli of a cellulase gene from Ruminococcus flavefaciens.
An endoglucanase gene of Ruminococcus flavefaciens FD1 was cloned on the vector pEcoR251 to form the recombinant plasmid pMEB200. The cloned endoglucanase gene showed carboxymethylcellulase enzyme activity but no degradation of Avicel (FMC Corp., Philadelphia, Pa.) or filter paper. Carboxymethylcell...
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| Udgivet i: | J Bacteriol |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1987
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC212014/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3549703/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.4.1760-1762.1987 |
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