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Use of TnphoA to detect genes for exported proteins in Escherichia coli: identification of the plasmid-encoded gene for a periplasmic acid phosphatase.

The structural gene (appA) for the periplasmic acid phosphatase (optimum pH 2.5) of Escherichia coli was cloned into a plasmid by using a combination of in vivo and in vitro techniques. The position and orientation of the appA gene within the cloned DNA fragment were identified by using fusions to t...

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Detalles Bibliográficos
Publicado en:J Bacteriol
Autores principales: Boquet, P L, Manoil, C, Beckwith, J
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology (ASM) 1987
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Acceso en línea:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211997/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3031017/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.4.1663-1669.1987
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