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Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.

We cloned the structural genes for the individual subunits of the branched-chain keto acid dehydrogenase multienzyme complex on a 7.8-kilobase EcoRI-SstI restriction fragment of Pseudomonas putida chromosomal DNA by cloning into the broad-host-range vector pKT230. A direct selection system for growt...

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Dettagli Bibliografici
Autori principali: Sykes, P J, Burns, G, Menard, J, Hatter, K, Sokatch, J R
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1987
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC211990/
https://ncbi.nlm.nih.gov/pubmed/3549697
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