Molecular cloning of genes encoding branched-chain keto acid dehydrogenase of Pseudomonas putida.

We cloned the structural genes for the individual subunits of the branched-chain keto acid dehydrogenase multienzyme complex on a 7.8-kilobase EcoRI-SstI restriction fragment of Pseudomonas putida chromosomal DNA by cloning into the broad-host-range vector pKT230. A direct selection system for growt...

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Pubblicato in:	J Bacteriol
Autori principali:	Sykes, P J, Burns, G, Menard, J, Hatter, K, Sokatch, J R
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1987
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211990/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3549697/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.4.1619-1625.1987
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