Cloning and nucleotide sequence of the gene coding for enzymatically active fragments of the Bacillus polymyxa beta-amylase.

The gene encoding beta-amylase was cloned from Bacillus polymyxa 72 into Escherichia coli HB101 by inserting HindIII-generated DNA fragments into the HindIII site of pBR322. The 4.8-kilobase insert was shown to direct the synthesis of beta-amylase. A 1.8-kilobase AccI-AccI fragment of the donor stra...

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Bibliografiske detaljer

Udgivet i:	J Bacteriol
Principais autores:	Kawazu, T, Nakanishi, Y, Uozumi, N, Sasaki, T, Yamagata, H, Tsukagoshi, N, Udaka, S
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1987
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211983/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2435707/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.169.4.1564-1570.1987
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