Cloning and nucleotide sequence of the gene coding for enzymatically active fragments of the Bacillus polymyxa beta-amylase.

The gene encoding beta-amylase was cloned from Bacillus polymyxa 72 into Escherichia coli HB101 by inserting HindIII-generated DNA fragments into the HindIII site of pBR322. The 4.8-kilobase insert was shown to direct the synthesis of beta-amylase. A 1.8-kilobase AccI-AccI fragment of the donor stra...

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Bibliographic Details
Main Authors: Kawazu, T, Nakanishi, Y, Uozumi, N, Sasaki, T, Yamagata, H, Tsukagoshi, N, Udaka, S
Format: Artigo
Language:Inglês
Published: 1987
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC211983/
https://ncbi.nlm.nih.gov/pubmed/2435707
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