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Ultrastructural visualization of cytoskeletal mRNAs and their associated proteins using double-label in situ hybridization

We have been able to visualize cytoskeletal messenger RNA molecules at high resolution using nonisotopic in situ hybridization followed by whole-mount electron microscopy. Biotinated cDNA probes for actin, tubulin, or vimentin mRNAs were hybridized to Triton-extracted chicken embryo fibroblasts and...

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Detalhes bibliográficos
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 1989
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2115600/
https://ncbi.nlm.nih.gov/pubmed/2738094
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