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Ultrastructural visualization of cytoskeletal mRNAs and their associated proteins using double-label in situ hybridization
We have been able to visualize cytoskeletal messenger RNA molecules at high resolution using nonisotopic in situ hybridization followed by whole-mount electron microscopy. Biotinated cDNA probes for actin, tubulin, or vimentin mRNAs were hybridized to Triton-extracted chicken embryo fibroblasts and...
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| Format: | Artigo |
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| Language: | Inglês |
| Published: |
The Rockefeller University Press
1989
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2115600/ https://ncbi.nlm.nih.gov/pubmed/2738094 |
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